Optical Structure of Phase Contrast Microscope

Optical structure of Compensating Microscope

Compensating microscope is an important instrument for metrology and physical measurements. Optical structure is the fundamental of a microscope, and how the microscope works also depends on its optical structure.

Optical structure of compensating microscope mainly consists of common optical elements, such as objective lens, compensation lens and eyepiece lens.

Objective lens is used to collect light from the measured object and produce a real image of the object. Generally, three familiar glass types are used to produce this image. Different lenses will be used depending on the corresponding application.

Compensation lens compensates for the shift in the optical axis of the existing objective lenses and corrective lenses, which reduces the amount of chromatic aberration, particularly spherical aberration, caused by turbulence in the entrance pupils of the lenses.

Eyepiece lens is placed at the end of compensating microscope system, which is a circular optical element with a working distance and a field of view. The eyepiece lens has very high resolution and can enlarge the real image produced by the objective lens to an easily observed size.

The optical structures of microscope compensate for the tilt of image when the telescope is moved, making the image have no tilt, which is called tilt compensation. The overlapping line formed by the objective lens of compensating microscope, with the small displacement of telescope, will have no shift, allowing observers to observe the smallest details at any position in the field of view.

The optical structure of compensating microscope has to be adjusted according to the measured object. The operating mechanism of the objective lenses, compensation lenses and eyepiece lenses needs to be adjusted, including setting the distance, position, inclination and focal length of the optical elements. The adjustment of objective lens can realize the observation of different distances in the same field of view. The main task of adjusting the compensating lens is to adjust the dark-field position of the objective lens. The main task of the eyepiece lens is to magnify and enable the observer to observe more stereoscopic details.

The accuracy of metering and physical measurement depends on the stability and accuracy of optical structure. Only when the optical structure meets the design requirements and works properly, can it provide reliable data support for further image analysis and interpretation. Therefore, the accuracy and stability of optical structure are important preconditions for compensating microscope working properly.

介绍

A compound microscope is an optical instrument that combines two or more lenses to increase the magnification of an object. It is composed of two main parts: an objective lens, which is placed close to the object, and an eyepiece that is placed near the eye of an observer. The objective lens gathers and focuses light from the object and produces a magnified image of the object, while the eyepiece magnifies the image further and allows the observer to view it. This type of microscope is widely used in biology and other scientific fields to study different samples in great detail.

In order to have a clear view of the sample being observed, the objective lens and the eyepiece lenses must be precisely aligned. This is known as the optical alignment, or collimation. This alignment must be checked before using the microscope, to ensure that the image seen through the eyepiece is correctly magnified and detailed. The optical structures that facilitate the optical alignment of the microscope lenses are known as the compensating or collimating lenses.

The compensating lenses are located between the objective and the eyepiece lenses and are used to adjust the amount of light that reaches the eyepiece, as well as to correct any misalignment between the two lenses. They also help to correct any image distortions that may occur due to the aberrations of the objective lens. Compensating lenses are usually made of tough optical glass with a high refractive index to ensure precise alignment of the two lenses.

The overall magnification provided by the microscope is dependent on the combination of the eyepiece and objective lens magnifications, as well as the compensating lens. The precise alignment of the compensating lenses is critical for providing unaltered images of the sample being observed. The use of the compensating lenses also helps to reduce the strain on the observer’s eyes, as a result of observing the magnified image for a long time. Therefore, it is important to make sure that the compensating lenses in a microscope are properly aligned and adjusted for optimum performance.