Structure Characteristics of Polarizing Light Microscope
Polarizing light microscope is also known as an optical microscope. It is a polarizing microscope that uses specific optical principles according to the polarization characteristics of reflected light to observe the transparency and birefringence under the light of certain materials in order to study their microscopic structural characteristics.
The polarizing light microscope optimizes the comparison microscope, polarimeter, and birefringent microscope by improving the condenser and objective system and adding a variety of accessories with strong automaticity. It can accurately display the shape, size and distribution of the object under observation, so as to identify and analyze the unknown object more accurately and quickly, and also show its microscopic structure information.
The research of polarizing light microscopes is mainly focused on the composition of natural products, and also on the birefringence and optical properties of various substances. It is widely used in the study of physics, chemistry, biology and mineralogy. And has a very important role.
The structure of the polarizing light microscope mainly includes a light source, an exciter polarizer, an analyzer polarizer, aanalyzer polarizer, a condenser system, an objective system, a rotating stage, eyepieces and a pointer. The source of illumination can be divided into two categories: sun-shaped and concentrated; for most observation of transparent and birefringent objects, the concentrated type should be used.
The condenser system includes a condensing lens, a condensing pole and a stop. The size of the stop is adjusted according to the amount of illumination, which is generally 18mm.
The objective system includes four objective lenses of different magnifications, which are 10×, 40×, 100×, and 400×. The eyepiece system includes three eyepieces of different magnifications, which are 10×, 15×, 20×.
In order to observe the image of the object under observation in the polarizing microscope, the exciter polarizer and analyzer polarizer should be used to generate two beams of light of the same strength and crossing the same optical axis.
The rotating stage is a mechanical device that is used to adjust the orientation of the object under observation in order to observe its optical properties from different directions.
The principle of the polarizing light microscope is based on the difference of optical path length. When the incident light passes through the birefringent object, it is split into two components of different refractive indexes, and the optical path length of these two components is different. The difference of the path length is used to produce the difference between the appearance of the two components of the incident light. This difference can be used to observe the structure and nature of the substance under test. For example, when the sample is rotated, the size and shape of the difference of the two components of the light will change, which provides a reliable means for the observation of the nature and structure of the material under test.
In summary, the polarizing light microscope has a wide range of applications in the study of the microscopic structure of various substances. The optical principles involved in this instrument can make it easier for us to observe and study the properties of materials and the microscopic structure of materials.
