，定位是针对gel permeation chromatography或者gel filtration chromatography，
Gel Permeation Chromatography (GPC) and Gel Filtration Chromatography (GFC)
Gel permeation chromatography (GPC) and gel filtration chromatography (GFC) are chromatography techniques used to separate macromolecules based on their size. The size of the macromolecules determines how they interact with the pores of the gel matrix, with smaller molecules able to penetrate deeper into the pore structure. By adjusting the size of the pores of the gel matrix, one can achieve a greater degree of separation between particles.
GPC utilizes a gel that is made up of polystyrene beads with pore sizes that can range from 5 angstroms to thousands of angstroms in size. These beads contain a hydrophobic groups that interact with the molecule being tested and the nonpolar parts of the molecule are excluded from the pore structure. As the pore size increases, so does the degree of steric selectivity, thus allowing for greater separation of macromolecules based on size.
GFC utilizes a gel matrix made up of cross-linked polysaccharides and proteins with pore sizes that can range from 500 angstroms to several microns in size. These pores are smaller than those used in GPC and so they provide a greater degree of separation between molecules based on their size. Furthermore, the hydrophilic nature of the gel matrix allows for the analysis of both hydrophilic and hydrophobic molecules.
The major advantage of GPC and GFC over other chromatographic techniques is their high resolution. GPC can be used to separate molecules based on a very small differences in size, whereas GFC can resolve molecules based on a difference of as little as 1 angstrom. This allows for a much more accurate analysis of the composition of the sample being tested.
Another advantage is that these techniques can be used to separate both large and small molecules. In addition, GFC enables the analysis of both aqueous and organic samples. This allows for a much more versatile approach when carrying out either an analytical or preparative style of chromatography.
Finally, one advantage of using these techniques is the cost effectiveness. GPC and GFC are relatively inexpensive when compared to other chromatographic techniques, making them an attractive choice for laboratories with limited budgets.
In conclusion, GPC and GFC are versatile and efficient chromatography techniques that can be used to separate both large and small molecules, with a high degree of accuracy and at a lower cost than other techniques. They are well suited for laboratories that require a low cost and versatile approach to sample analysis.
