Radioactive Selection
Radioactive selection is a method of gene selection in which a specific gene is identified and selected for further manipulation. The steps involved in the process are described below :
1. Codon-Based Selection: The first step of the Radioactive Selection process is to identify the specific codon that contains the genetic information for the desired gene. During this step, the codon sequence of a gene is compared to the known reference sequence in order to identify any variations or mutations in the genetic code.
2. Targeting: In order to isolate the desired gene, a molecule known as a targeting agent (TA) is used. It works by binding to the specific codon sequence that contains the desired genetic information. Once the TA is successfully bound to the codon sequence, it allows the specific gene to be isolated.
3. Isolation: Once the targeting agent is bound to the desired codon sequence, a DNA isolation process is initiated. This is typically followed by the use of a biochemical process to capture the selected gene before being isolated.
4. Sequencing: This step involves extracting the genetic information encoded within the selected gene and determining the exact sequence of DNA bases, in order to properly identify the specific gene.
5. Characterization: Characterization involves the analysis of the selected gene, in order to determine the protein product made from it and the potential role it may play in the organism from which it was isolated.
Radioactive selection is a process that is used to isolate a specific gene from a population of organisms or from a single organism. It is relatively simple procedure, but it requires careful consideration of the formulation of the targeting agent, the isolation and sequencing process, and the characterization of the components of the gene and its potential role. Once the selected gene is obtained, it can then be used for biotechnical applications such as bio-production or gene therapy.
